Description
StemmeraTM Human Blood Cell (hBLD) Reprogramming Kit is a ready-to-use and fully chemically defined reprogramming kit which composed of necessary reagents and tools intended for culturing human PBMCs and induction of pluripotent stem cells (hiPSCs) from PBMCs, as well as maintenance of iPSCs under completely serum-free and feeder-free conditions.
Features
- Ready-to-use for blood cell culture, blood cell reprogramming & freezing of iPSCs
- One-step reprogramming
- High efficiency reprogramming
- Serum/feeder-free culture and maintenance
- Easy protocol
Product Component
Human Blood Cell Reprogramming Kit-I
- Cat# ST01005-S1Non-Enzymatic Cell Dissociation Solution (100ml)
- Cat# ST01005-S2/BMBlood Cell Reprogramming Basal Medium (2x500ml)
- Cat# ST01005-S3DMEM/F12 (60ml)
Human Blood Cell Reprogramming Kit-II
- Cat# ST01005-S4/BMBlood Cell Basal Medium (2x25ml)
- Cat# ST01005-S4/SBlood Cell Supplement (61μl for 25ml of Basal Medium)
- Cat# ST01005-S5Polybreen (10μl)
- Cat# ST01005-S6Matrix (5×100μl)
- Cat# ST01005-S2/SBlood Cell Reprogramming Supplement (2×3.5ml)
- Cat# ST01005-S7Rock Inhibitor (1000x) (500μl)
- Cat# ST01005-S8Blood Cell CryopreservationSolution (10ml)
- Cat# ST01005-S9Serum-Free Cryopreservation Solution for hPSC (20ml)
Human Blood Cell Reprogramming Kit-III
- Cat# ST01005-S10-24Cell Culture Plates (1×24 well)
- Cat# ST01005-S10-12Cell Culture Plates(1×12 well)
- Cat# ST01005-S10-6Cell Culture Plates(4×6 well)
- Cat# ST01005-S11Cell Counter Slides (3pcs)
- Cat# ST01005-S12Trypan Blue (300μl)
Reprogramming Factors (Not supplied)
Note: Sendai virus reprogramming factors from Life Technologies Inc is strongly recommended, Cat # A16517
Storage & Handling
Store all components as directed in manual instruction upon arrival and until the expiration dates stated on the product label. Avoid multiple freeze/thaw cycles. The complete medium should remain stable for 4-6 weeks at 2-8°C. Make aliquot of complete medium for daily use, avoid warm up cycles. Protect from light is preferred.
Product Use
This product is intended for in vitro use and research use only. Not intended for human or animal diagnostic or therapeutic uses.
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