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Home Stemmera Kit Human Blood Cell (hBLD) Reprogramming Kit

Human Blood Cell (hBLD) Reprogramming Kit

$3,850.00

StemmeraTM Human Blood Cell (hBLD) Reprogramming Kit is a ready-to-use and fully chemically defined reprogramming kit which composed of necessary reagents and tools intended for culturing human PBMCs and induction of pluripotent stem cells (hiPSCs) from PBMCs, as well as maintenance of iPSCs under completely serum-free and feeder-free conditions.

Catalogue No.: ST01005
Pack size: 5 reactions

Category: Stemmera Kit
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Description
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Technical Resources
Data

Description

StemmeraTM Human Blood Cell (hBLD) Reprogramming Kit is a ready-to-use and fully chemically defined reprogramming kit which composed of necessary reagents and tools intended for culturing human PBMCs and induction of pluripotent stem cells (hiPSCs) from PBMCs, as well as maintenance of iPSCs under completely serum-free and feeder-free conditions.

Features

  • Ready-to-use for blood cell culture, blood cell reprogramming & freezing of iPSCs
  • One-step reprogramming
  • High efficiency reprogramming
  • Serum/feeder-free culture and maintenance
  • Easy protocol

Product Component

Human Blood Cell Reprogramming Kit-I

  • Cat# ST01005-S1Non-Enzymatic Cell Dissociation Solution (100ml)
  • Cat# ST01005-S2/BMBlood Cell Reprogramming Basal Medium (2x500ml)
  • Cat# ST01005-S3DMEM/F12 (60ml)

Human Blood Cell Reprogramming Kit-II

  • Cat# ST01005-S4/BMBlood Cell Basal Medium (2x25ml)
  • Cat# ST01005-S4/SBlood Cell Supplement (61μl for 25ml of Basal Medium)
  • Cat# ST01005-S5Polybreen (10μl)
  • Cat# ST01005-S6Matrix (5×100μl)
  • Cat# ST01005-S2/SBlood Cell Reprogramming Supplement (2×3.5ml)
  • Cat# ST01005-S7Rock Inhibitor (1000x) (500μl)
  • Cat# ST01005-S8Blood Cell CryopreservationSolution (10ml)
  • Cat# ST01005-S9Serum-Free Cryopreservation Solution for hPSC (20ml)

Human Blood Cell Reprogramming Kit-III

  • Cat# ST01005-S10-24Cell Culture Plates (1×24 well)
  • Cat# ST01005-S10-12Cell Culture Plates(1×12 well)
  • Cat# ST01005-S10-6Cell Culture Plates(4×6 well)
  • Cat# ST01005-S11Cell Counter Slides (3pcs)
  • Cat# ST01005-S12Trypan Blue (300μl)

Reprogramming Factors (Not supplied)
Note: Sendai virus reprogramming factors from Life Technologies Inc is strongly recommended, Cat # A16517

Storage & Handling
Store all components as directed in manual instruction upon arrival and until the expiration dates stated on the product label. Avoid multiple freeze/thaw cycles. The complete medium should remain stable for 4-6 weeks at 2-8°C. Make aliquot of complete medium for daily use, avoid warm up cycles. Protect from light is preferred.

Product Use
This product is intended for in vitro use and research use only. Not intended for human or animal diagnostic or therapeutic uses.

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Technical Resources

Product Name Document Type Download
Human Fibroblast (hFIB) Reprogramming Kit Manual
DMEM-F12 MSDS
Fibroblast Cryopreservation Solution MSDS
Fibroblast Growth Basal Medium & Supplement MSDS
Fibroblast Reprogramming Basal Medium & Supplement MSDS
Matrix MSDS
Rock Inhibitor MSDS
Serum-Free Cryopreservation Solution for hPSC MSDS
Trypan Blue MSDS

Data

Quality Control

This product is used to reprogram human PBMCs to iPSCs and maintain the pluripotency of human iPSCs under serum-/feeder-free culture condition. To ensure the quality, the following images represent human PBMCs in suspension and the pluripotent morphology of human iPSCs growing on Matrigel with StemmeraTM Human Blood Cell (hBLD) Reprogramming Medium.

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